Combined RSS Items
- Publication date: Available online 30 April 2024Source: Journal of ProteomicsAuthor(s): Na Song, Kai Cui, Liqun Zeng, Yanwu Fan, Ziwei Wang, Pingyu Shi, Wei Su, Haijun Wang
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.3c00403
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00066
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00051
- Increased accessibility of liquid chromatography mass spectrometry (LC-MS) metabolomics instrumentation and software have expanded their use in studies of dissolved organic matter (DOM) and exometabolites released by microbes. Current strategies to annotate metabolomes generally rely on matching tandem MS/MS spectra to databases of authentic standards. However, spectral matching approaches typically have low annotation rates for DOM. An alternative approach is to annotate molecular formula based on accurate mass and isotopic fine structure measurements that can be obtained from state-of-the-art ultrahigh […]
- The lipid kinase phosphatidylinositol 4 kinase III alpha (PI4KIII/PI4KA) is a master regulator of the lipid composition and asymmetry of the plasma membrane. PI4KA exists primarily in a heterotrimeric complex with its regulatory proteins TTC7 and FAM126. Fundamental to PI4KA activity is its targeted recruitment to the plasma membrane by the lipidated proteins EFR3A and EFR3B. Here, we report a cryo-EM structure of the C-terminus of EFR3A bound to the PI4KA-TTC7B-FAM126A complex, with extensive validation using both hydrogen deuterium exchange […]
- Quote from: agentfourty7 on April 12, 2023, 07:04:27 AMQuote from: drchrispook on March 28, 2023, 12:47:07 AMYeah, I don't like this. Instead I've worked out that converting your data to mzML means you can just add an RT shift to the time value…
- Lyme disease is a tick-borne, multisystem infection caused by the spirochete, Borreliella burgdorferi. Although antibodies have been implicated in the resolution of Lyme disease, the specific B cell epitopes targeted during human infections remain largely unknown. In this study, we characterized and defined the structural epitope of a patient-derived bactericidal monoclonal IgG (B11) against Outer surface protein C (OspC), a homodimeric lipoprotein necessary for B. burgdorferi tick-mediated transmission and early-stage colonization of vertebrate hosts. High-resolution epitope mapping was accomplished through […]
- Analyst, 2024, Accepted ManuscriptDOI: 10.1039/D4AN00100A, PaperMichael J. Davern, Gabrielle V. West, Clara M.A. Eichler, Barbara J. Turpin, Yue Zhang, Jason D. SurrattPer- and polyfluoroalkyl substances (PFAS) are manufactured chemicals that have been detected across the globe. Fluorotelomer alcohols (FTOHs) are one PFAS class commonly found in indoor air due to emissions…The content of this RSS Feed (c) The Royal Society of Chemistry
- Analytical ChemistryDOI: 10.1021/acs.analchem.4c00456
- Analytical ChemistryDOI: 10.1021/acs.analchem.4c01028
- Plants normally obtain the nitrogen required for growth through their roots, often after application of synthetic fertilizer to the soil, at great cost to the environment and climate. Inoculation of plant seeds with nitrogen-fixing bacteria is a promising alternative means of supplying plants the nitrogen they require in an environmentally friendly manner. When maize seeds inoculated with nitrogen-fixing Gluconacetobacter diazotrophicus (Gd) are grown for two weeks in a 15N2 air environment, nanoscale secondary ion mass spectrometry (NanoSIMS) imaging shows the […]
- Journal of Proteome ResearchDOI: 10.1021/acs.jproteome.4c00026
- Cryoconite holes (CHs) are biological hotspots with a high biogeochemical turnover rate, contributing significantly to the glacial ecosystem's overall carbon cycles and net fluxes. There is limited information regarding the composition of low molecular weight (LMW) molecules formed through the metabolic processes of cryoconite-dwelling microbes. These molecules constitute a substantial portion of the dissolved organic matter (DOM) within cryoconite holes. Here, we evaluated different solvents to extract low molecular weight (LMW) compounds for untargeted metabolomics using reverse phase liquid chromatography […]
- If you're just wondering if the guys in suits who say words like "depreciation" and "gestation lag" (and might seem like the sort of guys having fun in college while you were doing enzyme kinetic curves) are just making stuff up – they do appear to have some math to rely on here and there.This comes from what might be the worst name of an academic journal I've ever heard of, but it's funny how much (what I can understand […]
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00060
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00036
- Journal of the American Society for Mass SpectrometryDOI: 10.1021/jasms.4c00100
- Polyphosphate and tyrosine phosphorylation in the N-terminal domain of the human mitochondrial Lon protease disrupts its functions
- Abstract Antibiotic-associated diarrhea is a common adverse reaction caused by the widespread use of antibiotics. The decrease in probiotics is one of the reasons why antibiotics cause drug-induced diarrhea. However, few studies have addressed the intrinsic mechanism of antibiotics inhibiting probiotics. To investigate the underlying mechanism of levofloxacin against Bifidobacterium adolescentis, we used a metabolomics mass spectrometry-based approach and molecular docking analysis for a levofloxacin-induced B. adolescentis injury model. The results showed that levofloxacin reduced the survival rate of B. […]
- Vacuum-assisted headspace solid-phase microextraction (Vac-HS-SPME) is a technique used to enhance SPME sampling of semi-volatile organic compounds. Here, it was combined with a high-capacity SPME Arrow, which features a larger volume of extraction phase and a more rugged configuration than traditional extraction fibers. An in-depth assessment of the critical parameters was conducted to achieve optimal extraction of representative compounds from a model solid sample matrix (Ottawa sand). Operational fundamentals investigated included the types of seals needed to create a leak-free […]
- Disorders of lipid metabolism are a common cause of coronary heart disease (CHD) and its comorbidities. In this study, ultra-performance liquid chromatography–high-resolution mass spectrometry in data-independent acquisition (DIA) mode was applied to collect abundant tandem mass spectrometry data, which provided valuable information for lipid annotation. For the lipid isomers that could not be completely separated by chromatography, parallel reaction monitoring (PRM) mode was used for quantification. A total of 223 plasma lipid metabolites were annotated, and 116 of them were […]
- Introduction Distinguishing and categorizing the origin of garlic are highly significant, considering its widespread use as a flavoring agent. With billions of dollars annually in global trade, garlic is frequently susceptible to fraudulent practices. Methodology Paper spray ionization mass spectrometry (PS-MS) was employed to quickly analyze garlic samples from distinct geographic origins: China and Brazil. The so-generated PS-MS data were treated with metabolomic multivariate approaches, and the garlic samples from these different geographic regions were easily discriminated. Results Brazilian garlic […]
- Rationale The chemical constituents of traditional Tibetan medicines (TTM) can be identified using high-performance liquid chromatography and high-resolution mass spectrometry (HPLC–MS/MS) technique. However, the HPLC–MS/MS technique requires the sample to be pretreated and then separated using the specific liquid chromatography method, which is time consuming. This study developed a ballpoint electrospray ionization (BPESI) technique for analyzing the chemical constituents of Sbyor-bzo-ghi-wang. This technique is a simple and inexpensive method for the rapid identification of the chemical constituents of TTMs. Methods […]
- Rationale The study addresses the challenge of identifying RNA post-transcriptional modifications when commercial standards are not available to generate reference spectral libraries. It proposes employing homologous nucleobases and deoxyribonucleosides as alternative reference spectral libraries to aid in identifying modified ribonucleosides and distinguishing them from their positional isomers when the standards are unavailable. Methods Complete sets of ribonucleoside, deoxyribonucleoside and nucleobase standards were analyzed using high-performance nano-flow liquid chromatography coupled to an Orbitrap Eclipse Tribrid mass spectrometer. Spectral libraries were constructed […]